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PCR Genotyping Service

The Institute of Neuropathology has devoted a sizeable amount of work and resources to establishing an automated workflow - aided by a JANUS pipetting robot (PerkinElmer) - which allows to genotype animal biopsies efficiently and with a high throughput. Our efforts have been very successful, and this now enables us to offer our services to other researchers within the USZ.

1 PCR reaction = isolation of genomic DNA from an ear biopsy (transgenic or knockout animals) by specific primers, and analysis on a capillary electrophoresis system.

The PCR results (example), including images of the PCR reaction, are made available online on a password-protected platform.

Price list: 
PCR genotyping service: CHF 7-11/PCR

For each mouse line, a list of the applicable PCR reactions will be defined. For new PCR reactions, clients must supply the primer sequences along with a validated protocol.
Contact and support:
- PCR results: Jingjing Guo, jingjing.guo@usz.ch phone 0041 44 255 9419
- PCR results: Paulina Pawlak, paulina.pawlak@usz.ch phone 0041 43 25 39726
- Biopsy handling: Mirzet Delic, mirzet.delic@usz.ch
- Database, User-login: Andre Wethmar, andre.wethmar@usz.ch